Phosphorylation sites of higher stoichiometry are more conserved

Stoichiometry of photosynthetic phosphorylation.

The P/2eratio of noncyclic photophosphorylation was investigated in the light of recent reports that it is greater than one and may reflect the existence of two types of electron transport, a phosphorylating and a nonphosphorylating type, superimposed on each other. Our results, obtained under experimental conditions that included those reported to be conducive to high ratios, are consistent wi...

Conserved Orb6 Phosphorylation Sites Are Essential for Polarized Cell Growth in Schizosaccharomyces pombe

The Ndr-related Orb6 kinase is a key regulator of polarized cell growth in fission yeast, however the mechanism of Orb6 activation is unclear. Activation of other Ndr kinases involves both autophosphorylation and phosphorylation by an upstream kinase. Previous reports suggest that the Nak1 kinase functions upstream from Orb6. Supporting this model, we show that HA-Orb6 overexpression partially ...

Large-scale comparative phosphoproteomics identifies conserved phosphorylation sites in plants.

Knowledge of phosphorylation events and their regulation is crucial to understand the functional biology of plants. Here, we report a large-scale phosphoproteome analysis in the model monocot rice (Oryza sativa japonica 'Nipponbare'), an economically important crop. Using unfractionated whole-cell lysates of rice cells, we identified 6,919 phosphopeptides from 3,393 proteins. To investigate the...

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Telomeric Expression Sites Are Highly Conserved in Trypanosoma brucei

Subtelomeric regions are often under-represented in genome sequences of eukaryotes. One of the best known examples of the use of telomere proximity for adaptive purposes are the bloodstream expression sites (BESs) of the African trypanosome Trypanosoma brucei. To enhance our understanding of BES structure and function in host adaptation and immune evasion, the BES repertoire from the Lister 427...